Ribonucleotide Reductase and Cell Proliferation

Ribonucleotide reductase M1 subunit in cellular proliferation, quiescence, and differentiation.

Ribonucleotide reductase catalyzes the first unique, rate-limiting step in DNA synthesis; both its large (M1) and small (M2) subunits are necessary for activity. While direct studies of M2 expression have previously shown a tight correlation with S phase, the kinetic features of M1-expressing cells have remained ill defined. Therefore we have, using immunofluorescence flow cytometry, analyzed c...

Ribonucleotide Reductase Ml Subunit in Cellular Proliferation, Quiescence, and Differentiation

Ribonucleotide reducÃ-asecatalyzes the first unique, rate-limiting step in DNA synthesis; both its large (Ml) and small (M2) subunits are necessary for activity. While direct studies of M2 expression have previously shown a tight correlation with S phase, the kinetic features of Mi-expressing cells have remained ill defined. Therefore we have, using immunofluorescence flow cytometry, analyzed c...

Potent siRNA inhibitors of ribonucleotide reductase subunit RRM2 reduce cell proliferation in vitro and in vivo.

PURPOSE Ribonucleotide reductase (RR) is a therapeutic target for DNA replication-dependent diseases such as cancer. Here, a potent small interfering RNA (siRNA) duplex against the M2 subunit of RR (RRM2) is developed and shown to reduce the growth potential of cancer cells both in vitro and in vivo. EXPERIMENTAL DESIGN Three anti-RRM2 siRNAs were identified via computational methods, and the...

Ribonucleotide reductase and cell proliferation. I. Variations of ribonucleotide reductase activity with tumor growth rate in a series of rat hepatomas.

The relationship between ribonucleotide reductase and tumor growth rate was measured in a series of rat hepatomas of different growth rates. An excellent correlation was found between tumor growth rate and the specific activity of ribonucleotide reductase. Differences of ZOO-fold in enzyme specific activities exist between the very fast and the slow growing hepatomas. This represents the larges...

Ribonucleotide reductase in developing brain.